Conjunctival swabbing for the isolation of TRIC agent (Chlamydia).
نویسندگان
چکیده
Conjunctival scraping has been the standard method of collection of specimens for isolation of TRIC agent.* However, in spite of good instrumentation, even in the hands of experienced workers, conjunctival scraping commonly causes moderate or severe irritation, some bleeding, and conjunctival reaction, especially in acute or subacute conjunctivitis, and intensifies the patient's discomfort. It is therefore desirable to find an alternative and simpler method which would ensure a proper collection of specimens with minimal harm to tissues. Swabbing the conjunctiva has been used for isolation of TRIC agent by Tang, Huang, Chang, and Wong (I91;7 I958), Collier and Sowa (I958), Collier, Duke-Elder, and Jones (1958), and Gear, Cuthbertson, and Ryan (I967), but the technique has not come into general use. The purpose of this study was to investigate the efficiency of swabbing for the collection of specimens from the conjunctiva for the isolation of TRIC agent in comparison with a standard technique of scraping.
منابع مشابه
Isolation of Chlamydia spp. from Ewes and Does in Iran
Enzootic ovine abortion is caused by Chlamydia abortus and may result in abortion among small ruminants during the last 2-3 weeks of pregnancy. Enzootic abortion is diagnosed by isolation of the agent or detection of its nucleic acid in the products of abortion or vaginal excretions of freshly aborted females. Isolation of chlamydial agents in cell culture is the gold standard, so in the presen...
متن کاملIsolation of TRIC
The technique of cultivation ofTRIC agent, and other Chlamydia (Bedsonia) by centrifugation onto irradiated McCoy cells, developed by Gordon, Magruder, Quan, and Arm (i 963), Magruder, Gordon, Quan, and Dressler (i963), and Gordon, Dressler, and Quan (I967), has been used to recover laboratory-adapted isolates of TRIC agent from the infected eyes of simians (Gordon and others, I963) and of man ...
متن کاملIsolation of TRIC agent (Chlamydia) in irradiated McCoy cell culture from endemic trachoma in field studies in Iran. Comparison with other laboratory tests for detection of Chlamydia.
متن کامل
Antichlamydial antibody in tears and sera, and serotypes of Chlamydia trachomatis isolated from schoolchildren in Southern Tunisia.
A predominance of TRIC serotype A has been isolated from schoolchildren in a population in Southern Tunisia with severe hyperendemic trachoma. The serotyping results correspond precisely with the serological findings in patients' tears and sera. Geometric mean titres of serum or tear antibody in defined populations or areas can thus give a useful indication of the prevalent serotypes. Collectio...
متن کاملChlamydia trachomatis isolated from schoolchildren in Southern Tunisia
A predominance of TRIC serotype A has been isolated from schoolchiildren in a population in Southern Tunisia with severe hyperendemic trachoma. The serotyping results correspond precisely with the serological findings in patients' tears and sera. Geometric mean titres of serum or tear antibody in defined populations or areas can thus give a useful ind;cation of the prevalent serotypes. Collecti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The British journal of ophthalmology
دوره 55 9 شماره
صفحات -
تاریخ انتشار 1971